A team composed of the clinical medical school of Ningxia Medical University,the Department of Orthopaedics and spine orthopaedics of the General Hospital of Ningxia Medical University(Yinchuan,Ningxia Hui Autonomous Region),used the 3D printer and nano hydroxyapatite powder of Xi'an Particle Cloud Biotechnology Co.,Ltd.to prepare the 3D printed artificial bone loaded with anti tuberculosis drugs and bone morphogenetic protein 2 slow-release microspheres,By observing the effect of the artificial bone on the osteogenic differentiation and cell attachment ability of rabbit bone marrow mesenchymal stem cells,we judged its biocompatibility and osteogenic activity in vitro,and published the paper"3D printed artificial bone loaded with anti tuberculosis drugs and bone morphogenetic protein 2 slow-release microspheres can promote the osteogenesis of bone marrow mesenchymal stem cells"in China tissue engineering research,which was supported by the National Natural Science Foundation of China(8176090169).

The particle cloud biological 3D printer adopts the silk-free printing technology,supports the free proportioning of composite printing slurry,and accurately controls the drug release characteristics and mechanical strength of artificial bone.The 3D printed anti tuberculosis artificial bone loaded with new anti tuberculosis drug/bone morphogenetic protein 2/nano hydroxyapatite prepared in the previous experiment has a good spatial microstructure,which is conducive to cell attachment;As a drug delivery system with local anti tuberculosis and bone defect repaireffects,the drug loaded artificial bone was released in vitro for more than 6 weeks,and the average drug concentration was higher than 10 times the minimum inhibitory concentration.

Particle Cloud biological 3D printer

Hydroxyapatite is the main inorganic mineral component of animal and human bones,which has good biological activity and biocompatibility,ParticleCloud biological nano hydroxyapatite,nHA,a synthetic material similar to the main inorganic components of natural bone,can form a close combination with bone,non-toxic,non irritating,non allergic reaction,non mutagenic,non hemolytic,non damaging biological tissue,and can form a solid chemical combination with bone,which is easy to plastic.It is widely used in bone tissue repair.The product specification meets the pharmaceutical industry standard of the people's Republic of China YY 0303-1998 requirements of medical hydroxyapatite powder.

Particle Cloud hydroxyapatite

The experimental conclusion shows that the newly developed anti tuberculosis artificial bone loaded with new anti tuberculosis drug/bone morphogenetic protein 2/nano hydroxyapatite not only has good biocompatibility,but also can achieve good osteogenesis by promoting the proliferation and differentiation of bone marrow mesenchymal stem cells.It is an ideal bone tissue engineering material.Although it has satisfactory biocompatibility in vitro.It is more important to study its toxicity in vivo,especially in the focus of tuberculosis.In the future,we will further study the toxic side effects and osteogenic differentiation ability in animals,so as to lay a certain foundation for the treatment of tuberculous bone defects with tissue-engineered bone.

The development of biological 3D printing technology has provided new ideas and methods to a large extent.Although many scholars have achieved good results in the field of tissue engineering and alleviated the clinical pressure,there are still many problems need to be studied and verified.Xi’an Particle Cloud Biotechnology Co.,Ltd.is committed to the research and development of the core technology of 3D printing renewable artificial bone.In the future,it will continue to cooperate sincerely with college users,give full play to its own technology and equipment advantages,help colleges and universities carry out cutting-edge research on 3D printing technology,support college users'continuous exploration in the field of biological tissue engineering,and publish more valuable papers.